Figure 6

Alignment of TrpEb from S. typhimurium (Sty) with the TrpEb proteins from C. trachomatis (Ctr) and C. psittaci (Cps). Cps TrpEb1 is the paralog within the operon shown in Figure 1. Residues in the Sty sequence that are invariant in an extensive multiple alignment are marked in yellow and near-invariant residues are shaded in gray. Substrate, cofactor and metal-coordinating residues are color-coded as indicated by the key at bottom right. The movable COMM domain that interacts with the α subunit is marked, as well as residues K167 and S178 (not conserved) which contact the indicated residues of the α subunit. Residues critical for formation of internal salt bridges are also shown. See Xie et al. [30] for a detailed analysis. See Table 2 for organism acronyms and gene identification numbers.