Figure 4

Alignment of E. coli (Eco) TrpC from the protein database (PDB code 1PII) with TrpC proteins from the indicated chlamydiae (Cmu, C. muridarum; Cps, C. psittaci; Ctr, C. trachomatis). The TrpC domain shown for Eco TrpC is the carboxy-terminal domain of a fusion protein (TrpD•TrpC) whose amino-terminal domain is TrpD. An extensive multiple alignment of TrpC proteins was carried out, and several potential pseudogenes were excluded. The gaps shown reflect the gaps required to align the Eco•TrpC with other sequences in the latter alignment (available on request). Invariant residues are highlighted yellow, and near-invariant (one exception tolerated) residues are shaded gray. Active-site residues are marked with a red triangle and and residues interacting with ligand with blue dots [60]. See Table 2 for gene identification numbers.