Skip to main content

Table 2 Experimental evaluation of candidates by directional RT-PCR

From: Computational discovery of sense-antisense transcription in the human and mouse genomes

  Sense (-)antisense (-) Sense (+)antisense (+) Sense (-)antisense (-) Sense (+)antisense (+)
Candidates 0 6 7 26
Negative controls 1 17 0 0
  1. Summary of results of directional RT-PCR reactions on 39 candidates and 18 controls (see Figure 8 and Materials and methods for description of the assay). PCR primers were designed to amplify predicted regions of bidirectional transcription. Control primers were designed to amplify either non-overlap regions of candidate transcripts or randomly selected regions of non-candidate transcripts. Six candidate primer sets and 17 negative control primer sets were positive for only sense transcription over the regions queried. Thirty-three candidate primer sets and no negative control primer sets were positive for antisense transcription over the regions queried. Of these 33 sets, 26 were also positive for sense transcription in the same tissue.