Human members of the eukaryotic protein kinase family

Table 1 Variability tolerated at key residue positions within the EPK catalytic domain

The partial alignment (see Additional data files) was used to examine conservation among previously identified [52] highly conserved residues in EPK subdomains I-IX. The amino acids listed along the top of the table correspond to residues of PKA-Calpha (NP_002721): G1 = G51, G2 = G53, G3 = G56, K73, E92, D167, N172, D185, E209, D221. ^*The number of residues in Table 1 that differed from the consensus residue are listed first, along with the number of sequences informative at that residue. ^†The top two most commonly substituted amino acids at the given position and the number of occurrences of the substituted amino acid at that position. ^‡The 27 EPK structural families defined in SCOP were searched for examples of EPKs with non-canonical residues at any of these ten positions. G53 is substitued three times with S (CK2, Cal, Phk) and once with A (Dap). Residue D in subdomain VII is substituted with E in titin. E in subdomain VIII is substituted with N in CK1.

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