Table 1 PCR primer pairs used for RT-PCR confirmatory assays on S. mansoni gender-associated transcripts identified by cDNA microarray analysis
From: Identification of Schistosoma mansonigender-associated gene transcripts by cDNA microarray profiling
Accession number/gene name | PCR primer pairs | PCR product size |
|---|---|---|
AI111005 | 5' - CTT CCG GAT GTA GAG GAT TTG- 3' 5' - CGG GAT ATG CGT TTG GAC TAG- 3' | 301 bp |
AI110935 | 5' - CGT TAA CAG TGC ACA GAT GCC- 3' 5' - GGA ATC CGA CTA CAT CAT TTC- 3' | 182 bp |
N21956 | 5' - CAG AAG AAC GAA ACT AAT CAC- 3' 5' - TTC TGA TGA TAC GAT ACC GTG- 3' | 245 bp |
N21941 | 5' - ATC ATC ATT ATG GTA CAT CAT- 3' 5' - TGA ATT TCA ACG TAT ATT CTC- 3' | 236 bp |
AI111017 | 5' - AAG TGT CCC TGA CAC AAC AAC- 3' 5' - GCC TGT TTC ATT TTC ACC TTC- 3' | 137 bp |
R95512 | 5' - TCG TTT ATT AGG AGG AAG ATC- 3' 5' - GCT TTC TGC AGC TTC GAG ACG- 3' | 271 bp |
AA559631 | 5' - TGT TAC ATA TAG TGG TGA GGG- 3' 5' - CGA CAC TTA CTG CCT GGA TGG- 3' | 210 bp |
AA559678 | 5' - GGT ATA TTT CGA GAA TGT GAG- 3' 5' - CGC TTA GGT TAA TCA TGA ATG- 3' | 338 bp |
Alpha tubulin | 5' - GGC GGT GGT ACT GGT TCT GGG- 3' 5' - CAT TTA GCG CAC CAT CGA AGC- 3' | 291 bp |
Chorion | 5' - GAA ACA GTC ACT CAC ACT CG- 3' 5' - ATG GCT GGG TTT GTA AGT GC- 3' | 461 bp |