Figure 4

RT-PCR analysis confirmed gene expression results obtained by cDNA microarray hybridizations. A 1 μg sample of total RNA was used to prime cDNA synthesis for both adult male and female samples (in the presence or absence of reverse transcriptase; +SSRT or -SSRT). PCR primers (Table 1) were designed for cDNA clone IDs AI111005, AI110935, N21956, N21941, AI111017, R95512, AA59678, AA559631 as well as chorion and alpha-tubulin. PCR amplification was performed for 35 cycles (except for alpha-tubulin and clone AA559631: 23 cycles). Spot morphology and color intensity for each RT-PCR-verified clone, obtained from one representative cDNA microarray hybridization, are included for comparison (except for alpha-tubulin which is not present on the cDNA microarray). The sex-associated expression of each transcript, as classified by both techniques, is also indicated.