Figure 7

Imaginal discs share a similar expression profile but differ from differentiated larval tissues. (a) Comparison between first and third leg discs. An enlargement of a representative block out of 32 blocks on the microarray is shown. It produced mostly yellow spots on the superimposed red (Cy5 labeling) and green (Cy3 labeling) images, indicative of a high degree of similarity in the respective expression profiles. (b) In a comparison of wing disc and fat body, the same block contained mostly red and green spots, indicating a high degree of divergence. (c,d) Scatterplots of Cy3 and Cy5 intensities in comparisons (c) between leg discs and (d) between wing disc and larval fat body. In the leg comparison the spots are in close proximity to the bisector (CC 0.93, SD 0.38) with only a small number of genes induced in either the leg1 or leg3 disc. In contrast, spots are spread widely to both sides of the bisector for the wing-to-fat body comparison (CC 0.37, SD 1.17), indicating a large number of differentially expressed genes. The data points are color coded such that spots that are induced >1.74 fold in the Cy5 channel are colored red and those induced >1.74 in the Cy3 channel in green. Ratios within a threshold of 1.74 are represented in black.