Figure 5
Genes preferentially expressed in wing and eye-antennal discs. Amplified RNA from single discs of five individual larvae was used to carry out direct comparisons between a wing imaginal disc and an eye-antenna imaginal disc of the same larva (experiments 51, 88, 146, 155, 156, 157, 173 and 175). Whenever samples from one larva were used for more than one experiment, different left to right combinations of the individual discs were made. In two experiments, combinations of discs from two larvae (experiment 74) or pools of five larvae (ten wing and ten eye-antenna discs, experiment 98) were used. Cluster analysis was performed on the dataset with the requirement to show induction >1.74 (0.8 of the log2-transformed R/G ratio) in at least five experiments. One hundred and forty genes grouped into different subclusters. After removal of double hits or genes with inconsistent induction, 97 genes remained. Twenty-four genes were induced in the wing imaginal disc (red) and 73 genes in the eye-antennal disc (green). Black indicates lack of induction. Fold induction was calculated as an average induction in all experiments (column 3). The name, description and molecular or biological function is indicated as in Flybase [44] (columns 4 and 5). Some genes with known expression in the respective tissue are included in the clusters, such as apterous, engrailed and glass (see text). Of this set, 22 genes are uncharacterized and do not code for known protein domains. Note the high induction of CG9335 (16 fold) and CG11849 (6.9 fold) in the eye-antennal cluster. Genes marked in red were chosen for in situ experiments (see Figure 6).