Figure 2
Linear amplification is highly reproducible. (a) Denaturing gel electrophoresis shows the size distribution of aRNA obtained after two rounds of linear amplification of 1 ng poly(A)+ RNA. Lane 1, total RNA; lanes 2 and 3, products of independent amplifications; M, molecular-weight markers, with numbers indicating the approximate lengths in nucleotides. (b,c) Scatterplots of the Cy3 and Cy5 signal intensities from hybridizations of two probes derived from independent amplifications of embryonic or wing-disc RNA. Starting materials were (b) 1 ng embryonic poly(A)+ RNA and (c) left and right wing imaginal discs of one wandering third-instar larva. CC, correlation coefficient; SD, standard deviation. SDs were calculated on the normalized log2-transformed R/G ratios.