Conservation of 14-3-3 proteins. (a) A graph of percentage similarity derived from (b), which shows a multiple sequence alignment of 40 selected 14-3-3 isoforms. Highly conserved helices in (a) are in red and are indicated by numbers that correspond to helices in the crystal structure of Figure 3. The alignment in (b) was created using Clustal W . Residues in red are 100% conserved across all isoforms; residues in blue are highly conserved. These red and blue colors correspond to the red and blue regions of the crystal structure shown in Figure 3.