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Table 3 Control genes ranked in order of their expression stability*

From: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Neuroblastoma

Fibroblast

Leukocyte

Bone marrow

Normal pool

B2M

HMBS

ACTB

ACTB

B2M

RPL13A

B2M

HMBS

B2M

ACTB

ACTB

RPL13A

HPRT1

HMBS

YWHAZ

TBP

SDHA

SDHA

TBP

RPL13A

YWHAZ

TBP

TBP

SDHA

UBC

HMBS

ACTB

RPL13A

GAPD

TBP

UBC

UBC

GAPD

HPRT1

HPRT1

SDHA

YWHAZ

B2M

YWHAZ

HMBS

HPRT1 - GAPD

HPRT1 - GAPD

UBC - YWHAZ

UBC - RPL13A

SDHA - GAPD

  1. *Increasing from top to bottom; the two most stable control genes in each cell type, for example HPRT1 and GAPD in fibroblasts, cannot be ranked in order because of the required use of gene ratios for gene-stability measurements.