Expression patterns of androgen-responsive genes in LNCaP cells exposed to R1881, DHT, or androgen deprivation. (a) Gene-expression changes in LNCaP treated with 1 nM R1881 and with dihydrotestosterone (DHT) at 10 nM, 100 nM or 1,000 nM. Note the apparent increase in gene expression with successively higher doses of DHT. On the far right are two experiments in which LNCaP cells were deprived of androgen for 48 and 72 h and that display reciprocal expression patterns. Green, red, black and gray bands as in Figure 1. Color-saturation scale as in Figure 1. (b) Correlation of gene-expression levels for LNCaP treated with 1 nM R1881 for 24 h and 1,000 nM DHT for 24 h. (c) Inverse correlation of gene-expression levels for LNCaP after treatment with 1 nM R1881 for 50 h and androgen deprivation for 72 h.