Table 3 Primers used for the generation and verification of yeast single knockouts

Primer

Sequence 5' to 3'

   YGR038w F

AGG AAC TGC TGA GGC GGC TTC TAC CTC GTA TAG TCG AAA TCA AAC CGT ACG CTG CAG GTC GAC

   YGR038w R

TGT TCA ACT AAT TTG GGC GCG ACC TGT GAT ACC TGG GAT AGA AAT ATC GAT GAA TTC GAG CTC G

   YLR350w F

ATC ATG ATT GAC CGC ACT AAA AAC GAA TCT CCA GCT TTT GCG TAC GCT GCA GGT CGA C

   YLR350w R

CTA ACT AAT TTG AGC ACG GCC CGT AAT ACC AGG GAT GGA TAT CGA TGA ATT CGA GCT CG

Oligonucleotides for gene integrity and gene deletion verification

Verification of ORM1 gene integrity

   A1 (F)

GTT TAG GAA CGG ATT ATA GA

   A2 (R)

CTG GTC ATT TTC ATC TTC AA

Verification of ORM1 substitution

   A1 (F)

GTT TAG GAA CGG ATT ATA GA

   KAN5 (R)

GTT CGG ATG TGA TGT GAG

Verification of ORM2 gene integrity

   YLR350w up400 (F)

GCG TAT TTT GAT TGC TCA TA

   YLR350w ver (R)

TGG TTT CAG GTT AGA CAC AT

Verification of ORM2 substitution

   YLR350w up400 (F)

GCG TAT TTT GAT TGC TCA TA

   KAN5 (R)

GTT CGG ATG TGA TGT GAG