Qualitative phenotypic analysis of yeast knockout strains and functional complementation of human ORMDL3. (a) Genotype PCR analyses of spores derived from tetrad dissection. The deduced genotype for each clone is indicated above. Four different PCR reactions were performed to assess the following: lane 1, ORM1 integrity; lane 2, ORM1 deletion; lane 3, ORM2 integrity; lane 4, ORM2 deletion. (b) Dropout plate showing the growth of haploid wild-type, single-knockout and double-knockout strains. (c) Growth of wild-type, single-knockout and double-knockout strains as well as functional complementation of double knockouts transformed with human ORMDL3. pSC16-hORMDL3 and pVT-U-hORMDL3 denote, respectively, yeast centromeric and non-centromeric plasmid constructs used in the complementation assay. Five sequential dilutions (1:5 each) were plated, left to right, in YPAD medium supplemented with the toxic agents at the indicated concentrations.