Whole-mount in situ hybridization to detect DORMDL expression in Drosophila embryos and imaginal discs. DORMDL is ubiquitously expressed in the ectodermal tissues. (a) Syncytial blastoderm (stage 3/4, approximately 2 h); (b) cellular blastoderm focused in the plane where polar cells are visible (stage 5, 3 h-3 h 15 min); (c) lateral view of an stage 11 embryo with the fully stretched germ band; (d) lateral view of an stage 11-12 embryo when the germ band begins to recede; (e) lateral view of a stage 14 embryo; (f) sense probe (stage 13). A, anterior; P, posterior; D, dorsal; V, ventral; pc, polar cells; cel, cellularization; pro, procephalon; gb, germ band; T1-T3, thoracic segments; A1-A9, abdominal segments. Timings of developmental stages are approximate (development at 25°C). (g) Eye-antenna imaginal disc; (h) leg imaginal disc; (i) wing imaginal disc; (j) negative control - wing imaginal disc hybridized with the sense probe.