Figure 1

HeLa cells stably expressing GFP-SMN showing localization of GFP-SMN (green) to the cytoplasm and to Cajal bodies in the nucleus. Cells were fixed with paraformaldehyde and stained with the monoclonal antibody Y12, which recognizes Sm proteins found in splicing speckles and Cajal bodies (red) and with DAPI, which recognizes bulk chromatin (blue). The scale bar represents 10 μm.