Figure 1

Cluster analysis of 161 genes whose expression in challenged monkey lungs changed >2.5-fold in at least one of the experimental conditions. (a) Cluster image of microarray expression profiles of 161 genes. The genes were clustered hierarchically into groups by the program S-plus on the basis of the similarity of their expression profiles. The expression pattern of each gene is displayed as a horizontal strip. The ratio of mRNA levels in allergen-challenged versus control unchallenged monkey lungs is represented by a color, according to the color scale shown (red, overexpression; green, underexpression). (b) Expression profile of individual genes in each group of the cluster analysis in (a). Each treatment group (4 h, 18 h, and 24 h after A. suum challenge and 24 h after IL-4 challenge) is an independent point; the connecting line is only for the purpose of easy visualization of the individual genes. (The solid line connects 4 h, 18 h and 24 h; the dotted line connects 24 h and IL-4). Only representative known genes are displayed from each cluster. In some clusters, a single gene is represented by multiple cDNAs. In each plot, a horizontal line is drawn at fold value = 1, which indicates equal expression in control and allergen-challenged monkey lungs. Points above this line represent fold overexpression in corresponding allergen-challenged monkeys, and points below this line represent fold underexpression (see Materials and methods).