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Table 3 Comparison of change folds between oligonucleotide arrays and RT-PCR

From: Evolutionary conservation of otd/Otx2 transcription factor action: a genome-wide microarray analysis in Drosophila

    

Change fold

 

Avg Diff

hsotd

hsOtx2

Transcript

hswt

hsotd

hsOtx2

Array

RT-PCR

Array

RT-PCR

scra

251

1375

1229

5.5

1.3

4.9

1.6

LysD

525

1646

2436

3.1

1.6

4.6

4.0

glu

479

1196

1991

2.5

1.8

4.2

10.9

Rpd3

1170

2562

2673

2.2

2.0

2.3

2.5

pim

118

246

1467

2.1

1.4

12.4

8.0

n-syb

612

293

296

-2.1

-1.5

-2.1

-1.5

eyg

1552

229

10

-6.7

-1.4

-77.6

-1.6

wun

885

/

884

/

/

1.0

1.0

Scc1

724

723

/

1.0

1.0

/

/

otd

84

6555

108

78.0

119.4

1.3

1.5

  1. RT-PCR was carried out on cDNA derived from heat-shocked wild type (hswt), heat-shocked otd (hsotd) or heat-shocked Otx2 (hsOtx2) embryos. Change folds determined by RT-PCR are represented as the mean value of eight independent replicates, derived from two different cDNA preparations. wun is used as a control for the comparison of the RT-PCR data between heat-shocked wild type and heat-shocked Otx2. Scc1 is used as a control for the comparison of the RT-PCR data between heat-shocked wild type and heat-shocked otd.
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