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Table 3 Comparison of change folds between oligonucleotide arrays and RT-PCR

From: Evolutionary conservation of otd/Otx2 transcription factor action: a genome-wide microarray analysis in Drosophila

     Change fold
  Avg Diff hsotd hsOtx2
Transcript hswt hsotd hsOtx2 Array RT-PCR Array RT-PCR
scra 251 1375 1229 5.5 1.3 4.9 1.6
LysD 525 1646 2436 3.1 1.6 4.6 4.0
glu 479 1196 1991 2.5 1.8 4.2 10.9
Rpd3 1170 2562 2673 2.2 2.0 2.3 2.5
pim 118 246 1467 2.1 1.4 12.4 8.0
n-syb 612 293 296 -2.1 -1.5 -2.1 -1.5
eyg 1552 229 10 -6.7 -1.4 -77.6 -1.6
wun 885 / 884 / / 1.0 1.0
Scc1 724 723 / 1.0 1.0 / /
otd 84 6555 108 78.0 119.4 1.3 1.5
  1. RT-PCR was carried out on cDNA derived from heat-shocked wild type (hswt), heat-shocked otd (hsotd) or heat-shocked Otx2 (hsOtx2) embryos. Change folds determined by RT-PCR are represented as the mean value of eight independent replicates, derived from two different cDNA preparations. wun is used as a control for the comparison of the RT-PCR data between heat-shocked wild type and heat-shocked Otx2. Scc1 is used as a control for the comparison of the RT-PCR data between heat-shocked wild type and heat-shocked otd.