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Table 8 Dyad-analysis/sweeping predictions in regions without binding sites reported in RegulonDB

From: Evaluation of thresholds for the detection of binding sites for regulatory proteins in Escherichia coliK12 DNA

Regulatory protein and genes shown to be regulated by it

Above threshold

Site coordinates and number of matches

Site sequence

ArcA (12)

   

aceB

-

i:-144;f:-85;m:8

TTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAG

fadB

-

i:-96;f:-51;m:6

ATTTCTTTAATCTTTTGTTTGCATATTTTTAACACAAAATACACAC

fumA

+

i:-77;f:-56;m:14

TATTGTTACTCGCTTTTAACAG

fumC

-

i:-92;f:-53;m:10

ATTTGTTATCAAATGGTAAATAATAAGTGAGCTAAAAGTT

glpA

-

i:-248;f:-232;m:8

TTATTTATGATTAACAG

hyaA

-

i:-168;f:-150;m:12

TACGCTTTATTAACAATAC

lpdA

+

i:-232;f:-204;m:15

TGTTTAAAAATTGTTAACAATTTTGTAAA

sucA

-

i:-323;f:-300;m:5

TGTTGTTGCAACGTAATGCGTAAA

ArgR (11)

   

argD

-

i:-63;f:-51;m:5

TTTTTATGCATAT

FNR (4)

   

aspA

-

i:-156;f:-144;m:3

TGATCTATTTCAC

cyoA

+

i:-25;f:2;m:5

GATCCCGTGGAATTGAGGTCGTTAAATG

icdA

+

i:-306;f:-292;m:6

ATTGAACAGGATCAC

sdhC

-

i:-340;f:-326;m:2

GATGATTAAAAATTA

LexA (9)

   

umuD

+

i:-57;f:11;m:24

CTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTAT

   

GTTGTTTATC

Lrp (1)

   

livK

+

i:-277;f:-269;m:2

CAGCATAAT

sdaA

-

-

-

serA

-

i:-146;f:-139;m:1

CAGCATAT

NarL (1)

   

adhE

-

i:-215;f:-201;m:1

TACCCAGAAGTGAGT

caiF

-

-

-

torC

+

i:-209;f:-195;m:2

TACCCCTCCTGAGTG

PurR (15)

   

codB

+

i:-82;f:-64;m:16

ACGAAAACGATTGCTTTTT

prsA

+

i:-356;f:-344;m:21

GAAAACGTTTTCG

speA

-

i:-132;f:-119;m:6

GAAACCGGTTGCGC

  1. Sequences and positions of binding sites predicted by dyad sweeping in genes with experimental evidence for co-regulation in RegulonDB, but with no binding site experimentally identified. Genes follow the alphabetic order of the regulatory proteins, with the name of the protein separating each group. The number in parentheses after the regulator is the value of the threshold - derived from requesting best overall performance. The site coordinates are 'i' for initial base, 'f' for final position relative to the start codon. The score is given as the maximum number of matching ('m') dyads within a ROM. The number of families used was the same for any method, but we only show families where the methods provided significant results.