Figure 4
Spiddo assignment. (a) A genome profile before processing. The temperature gradient is set from left (low) to right (high) and the direction of migration is top to bottom; IR, internal reference band used for normalization. (b) The spiddos of the genome profile are marked with red filled circles; those of the IR are indicated with red open circles. All the spiddos except for the rightmost one are at the first transition of DNA melting (Pini). Although there are four kinds of spiddos (dots), as described in Materials and methods, Pini is used for simplicity as these points are clearly visible.