Figure 8

The boundaries of the centric heterochromatin defined by FISH. BACs near the centric ends of the Release 3 chromosome arm sequence spanning the euchromatin [47] were localized by FISH to mitotic chromosomes to correlate the cytological boundaries of the centric heterochromatin with the genomic sequence (see Materials and methods). (a) Results for chromosome 3 are shown. Locations of BACs on the cytogenetic map (bands h1-61) are indicated by arrows. The left (3L) and right (3R) arms, and the centromere (C), are indicated. BAC names are indicated below each image, and images are oriented with the left arm at the top. See Table 1 for complete BAC names and additional information. (b) An example of the quantitative analysis used to determine BAC locations (red and green) relative to the DAPI (blue) banding pattern is shown (see Materials and methods).