Figure 2

Topology of the putative regulatory elements identified in 17 cardiac promoters. Gray boxes are polymerase II promoter elements at correct locations. TRANSFAC transcription factor sites and MEME motifs (arrows) are colored. Eight transcription factor sites are common to all sequences and their combination is represented by X. They are AP-2 and AP-4 (activator protein), SP1 (stimulating protein), NFAT (nuclear factor of activated T cells), the GC box, NF1_Q6 (nuclear factor of activated T cells), TCF11 (zinc-finger protein) and MZF1 (myeloid zinc finger). Brown squares are a transcription factor site (for GKLF (gut-enriched Krüppel-like factor)) located at the same position (-70) in at least 15 promoters. Green and orange symbols are couples of motifs that are separated by a constant width and are present in the maximum number of promoter sequences (seven and eight respectively). Both couples involve the TCF11 zinc finger with RORA (RAR-related orphan receptor alpha1) or AP1_C (activator protein 1). Blue arrows are MEME combinations of motifs. Light-blue arrows are the combinations of motifs M2-M1-M5 (M2 and M1 on the strand S+ and M5 on the strand S-, or vice versa). Dark-blue arrows are the combination of motifs M1-M3-M2-M6 (M1 and M2 on S+ and M3 and M6 on S-, or vice versa). Consensus sequences of the motifs are: M1: TC [CTA] [TG]G [GA]G [TG]CTGAGG [GC] [AG] [GA]GAGG [AG] [GT] [CT] [TG] [GC] [GT] [GT]G; M2: [AC] [CG] [TA]CCAGCCTGGG [CT] [GA]ACA [GT]AG [CT] [GA]A [GA]A [CT]CC [TC] [GT]T [CT]T [CT] [AT]A [AC] [AT]AAAA [AC] [AT] AA [AT]AA; M3: GTGG [CT]G [CT]GATC [AT] [CT]GGCTCACTGCA [GA]CCTC [GC] [AG]CCT [CT]C [CT]G; M5: GCTGGGA [TC]TACAGGC [GA]TG [AC]GCCAC [CG] [GA] [TC]GC; M6: TTT [TAG] [AT] [TA]TT [AT] [AT]TT [TC]TT [TC] [CA] [TG] [TC]T [TA] [TAG] [CT] [TA] [CT] [TA] [TG] [AC]C. The figure is not to scale.