Skip to main content

Table 2 RNAi with C. elegans ubiquitin-activating enzyme genes and ubiquitin-like genes

From: Functional and phylogenetic analysis of the ubiquitylation system in Caenorhabditis elegans: ubiquitin-conjugating enzymes, ubiquitin-activating enzymes, and ubiquitin-like proteins

Cosmid

Gene name

dsRNA source

Chromosome

Number of RNAi trials

Embryonic lethality (%)

Secondary phenotype

Ubiquitin-activating enzymes

      

   F11H8.1

uba-3

gen PCR

III

2

41

Evl

   C26E6.8

ula-1

yk433e12

III

2

6

Evl

   W02A11.4

uba-2

yk632h1

V

2

40

Evl

   C47E12.4

uba-1

yk9g12

IV

2

100

Low brood size, death

   C08B6.9

aos-1

-

V

Not done

-

-

Ubiquitin-like fusion proteins

      

   B0303.4

 

yk490f10

III

2

0

None

   C16C8.4

 

yk262b4

II

2

0

None

   F49C12.9

 

yk162f5

IV

2

0

None

   ZK688.5

 

yk16d10

III

2

2.5

None

Small ubiquitin-like proteins

      

   F45H11.2

ned-8

T01966

I

1

2

Evl, abnormal morphology

   K12C11.2

sumo

yk222g1

I

2

100

Evl, abnormal morphology

   F46F11.4

 

gen PCR

I

2

0

None

   F32H5.3

 

yk534f3

V

2

0

None

  1. DNA fragments used to generate the dsRNA were obtained from the indicated sources, some fragments were generated by genomic PCR (gen PCR). Phenotypes include Evl (everted vulva). A dash indicates experiment not done.
Back to article page

Genome Biology

ISSN: 1474-760X

Contact us