Table 1 Dissimilarity (D) comparison results from 50 validation sequences

chitin synthase Gvchs1

n

638

2,535.2

2,468.6

2,718.4

chitin synthase Gvchs2

n

481

2,203.2

2,050.0

2,286.0

chitin synthase Gvchs3

n

4,116

7,205.9

5,235.8

5,985.8

phosphate transporter

y

1,833

3,937.9

5,702.3

6,514.3

actin SAc3

n

1,620

3,322.0

4,554.6

5,329.7

lectin Le1

n

2,152

4,124.6

6,558.3

7,928.3

iron superoxide dismutase

y

1,056

2,773.6

3,761.2

4,269.2

phosphate transporter MtPT1

y

1,920

3,800.3

5,630.7

6,654.2

phosphate transporter MtPT2

y

1,867

3,673.9

5,390.1

6,424.0

Mt4 genomic sequence

n

954

2,631.9

4,004.4

4,539.1

cell wall protein AM1

y

885

3,433.6

4,200.0

4,774.3

translation initiation protein AM3-1

y

3,154

4,557.6

5,982.7

7,212.8

translation initiation protein AM3-2

y

1,384

3,371.1

4,130.0

4,644.4

ha1 gene, exons 1–22

n

3,620

4,383.2

8,683.6

10,730.7

MtNo213 superoxide dismutase

y

530

2,110.2

2,219.8

2,367.0

MtNo233 triosephosphate isomerase

y

563

2,171.6

2,405.6

2,618.6

peroxidase precursor rip1

n

2,603

4,246.1

8,210.0

9,901.9

sugar transporter

y

1,728

3,619.6

5,128.4

5,976.5

leghemoglobin Mtlb1

n

1,073

3,021.3

5,029.1

5,845.9

leghemoglobin Mtlb2

n

592

2,045.9

3,156.0

3,568.2

lectin lec1

n

1,363

3,228.8

4,935.4

5,605.6

lectin lec2

n

1,192

3,142.8

4,472.6

4,985.9

lec3

n

1,155

2,928.4

4,283.3

4,930.8

ENOD12

n

772

2,780.4

3,679.7

4,096.5

ENOD16

n

1,142

3,124.6

4,535.5

5,156.2

ENOD20

n

1,405

4,003.6

5,294.7

5,966.7

glutamine synthetase

y

1,413

3,116.1

4,506.5

5,292.1

chitinase

y

1,305

3,369.5

4,090.4

4,703.4

surface glycoprotein elicitor inf2A

y

648

3,428.4

2,421.9

2,589.1

surface glycoprotein elicitor inf2B

y

701

3,611.7

2,514.5

2,698.6

ipiO2

n

1,556

4,125.2

4,339.5

4,855.5

ipiO1

n

1,826

4,360.5

4,580.9

5,259.0

ipiB1

n

1,726

6,086.7

4,584.3

5,159.3

actin actA

n

1,736

5,137.1

3,637.2

4,420.0

actin actB

n

1,405

4,425.3

3,569.5

4,141.6

calmodulin calA

n

1,358

4,063.0

3,724.9

4,138.1

tigA

n

2,448

6,221.0

4,193.8

5,181.9

chitin synthase chs

n

449

2,238.8

1,882.7

1,997.5

cellulose-binding-elicitor lectin

y

918

3,819.1

2,876.0

3,208.4

nodD

n

1,776

5,317.9

4,197.8

4,179.4

superoxide dismutase sodA

n

1,196

4,898.2

3,343.2

2,916.0

exoD

n

858

4,071.8

2,847.2

2,372.9

nodulation proteins nodP and nodQ

n

3,476

9,992.3

5,288.0

3,954.7

phosphate regulators phoU and phoB

n

1,178

5,332.7

3,359.3

2,866.4

syrA

n

1,102

4,176.2

3,375.8

3,220.8

nodA, nodB, and nodC

n

3,373

7,684.1

4,819.5

4,646.1

regulatory nitrogen fixation fixD

n

2,111

5,723.0

4,249.8

4,228.3

glutamine synthetase II

n

990

4,303.5

2,959.9

2,720.4

putA

n

3,804

13,623.3

6,376.5

4,212.0

sugar transporter gguA and membrane-spanning permeases gguB and gguC

n

4,185

11,132.6

5,959.4

4,551.4

sugar transporter gguA and membrane-spanning permeases gguB and gguC