Figure 4

Overview of the mapping of one mutant. Step 1: The mutant was mapped between the visible markers, using ten recombinants in each interval, and SNP-RFLPs were used to refine the map position. Step 2: We collected more recombinant males in the previously determined visible interval and tested them with the RFLPs flanking the mutation, as determined in the first step. This allowed us to find 45 informative recombinants. Step 3: These recombinants were further tested with SNPs from the map. This placed our mutation in a 400 kb interval, with eight recombinants still available. Step 4: The last step involved the discovery of more SNPs in that same interval and the fine mapping of the gene. We were able to map it to a 47 kb region. The numbers below the schematic maps of the chromosome indicate the numbers of recombinants in the given intervals.