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Table 6 Bacterial strains and plasmids

From: The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa

Strain or plasmid Genotype or description Source or reference
Escherichia coli   
   BL21(DE3) F-ompT hsdSB (rB- mB-) gal dcm; with DE3, a λ prophage carrying the T7 RNA polymerase gene Novagen
   BL21(pJB-1) BL21(DE3) transformed with pJB-1 This study
   DH5α F-φ 80dlacZΔM15 Δ (lacZYA-argF) U169 deoR recA1 endA1 up44gλ-hsdR17 (rK-, mk+) thi-1 gyrA96 relA1 Gibco-BRL
Salmonella typhimurium   
   CAB101 tyrA19 [48]
   ATCC 15277 Prototroph ATCC
Klebsiella pneumoniae   
   62–1 (ATCC 25306) tyrA pheA trpD ATCC
Pseudomonas aeruginosa   
   PAT1051 aroQpheA (leaky phenylalanine auxotroph) [49]
   PAO1 Wild-type prototroph B.W. Holloway
Plasmids   
   pET-24b(+) T7 lac promoter, lacI+ Kmr HisTag sequence, T7Tag (11aa) sequence Novagen
   pJB-1 pET-24b(+) containing S. typhimurium*aroQ insert This study