Table 6 Bacterial strains and plasmids
Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
Escherichia coli | Â | Â |
   BL21(DE3) | F-ompT hsdSB (rB- mB-) gal dcm; with DE3, a λ prophage carrying the T7 RNA polymerase gene | Novagen |
   BL21(pJB-1) | BL21(DE3) transformed with pJB-1 | This study |
   DH5α | F-φ 80dlacZΔM15 Δ (lacZYA-argF) U169 deoR recA1 endA1 up44gλ-hsdR17 (rK-, mk+) thi-1 gyrA96 relA1 | Gibco-BRL |
Salmonella typhimurium | Â | Â |
   CAB101 | tyrA19 | [48] |
   ATCC 15277 | Prototroph | ATCC |
Klebsiella pneumoniae | Â | Â |
   62–1 (ATCC 25306) | tyrA pheA trpD | ATCC |
Pseudomonas aeruginosa | Â | Â |
   PAT1051 | aroQpheA (leaky phenylalanine auxotroph) | [49] |
   PAO1 | Wild-type prototroph | B.W. Holloway |
Plasmids | Â | Â |
   pET-24b(+) | T7 lac promoter, lacI+ Kmr HisTag sequence, T7Tag (11aa) sequence | Novagen |
   pJB-1 | pET-24b(+) containing S. typhimurium*aroQ insert | This study |