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Table 5 Kinetic constants, pI and Mw of *AroQ enzymes

From: The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa

    pI/Mr
Organism Km (μM) kcat (s-1) Uncleaved Cleaved
Pseudomonas 98 6.4 8.77 / 20,417 8.04 / 17,939
aeruginosa     
Salmonella 142 8.9 7.02 / 20,646 6.58 / 18,392
typhimurium     
Erwinia 169 9.7 6.28 / 20,299 6.24 / 18,017
herbicola     
Yersinia    6.43 / 20,761 5.66 / 17,504
pestis     
Mycobacterium    5.40 / 21,945 5.03 / 18,475
tuberculosis     
Meloidogyne    6.58 / 22,087 5.90 / 19,460
javanica     
  1. Reaction kinetics were monitored at 32°C using 40–75 nM enzyme purified in this study (P. aeruginosa and S. typhimurium) or previously (E. herbicola [12]). Bovine serum albumin was present at 0.1 mg/ml. Initial rates were used to calculate the steady-state kinetic parameters of Km and kcat. pI/Mr valueswere determined by use of the compute pI/Mw tool at ExPASy [47].