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Table 4 Purification of *AroQ from Pseudomonas aeruginosa

From: The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa

Purification Total protein Specific Purification Yield
  (mg) activity* (fold) (%)
Shock fluid 240 17.7 1 100
Hydroxylapatite 39.9 39.2 2.2 36.8
Mono Q (pH 7.0) 10.5 53.1 3 13.1
Mono Q (pH 8.4) 1.7 116.5 6.6 4.7
Superdex 75 0.44 487.5 27.5 5.0
  1. *Specific activity expressed as nmol/min/mg.