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Table 4 Purification of *AroQ from Pseudomonas aeruginosa

From: The emerging periplasm-localized subclass of AroQ chorismate mutases, exemplified by those from Salmonella typhimurium and Pseudomonas aeruginosa

Purification

Total protein

Specific

Purification

Yield

 

(mg)

activity*

(fold)

(%)

Shock fluid

240

17.7

1

100

Hydroxylapatite

39.9

39.2

2.2

36.8

Mono Q (pH 7.0)

10.5

53.1

3

13.1

Mono Q (pH 8.4)

1.7

116.5

6.6

4.7

Superdex 75

0.44

487.5

27.5

5.0

  1. *Specific activity expressed as nmol/min/mg.
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Genome Biology

ISSN: 1474-760X

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