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Table 2 Fold differences in gene expression in human Th1/Th2 cells

From: Distinct gene expression profiles of human type 1 and type 2 T helper cells

   Oligonucleotide Real-time RT-PCR       
   array        
Preference Gene Fold difference Fold difference   SD   SD Fold Statistical
     deltaCT (Th1)   deltaCT (Th2)    
   by microarray† by real-time (average of   (average of   difference† significance§
   (individual 1)‡ RT-PCR† individuals   individuals   (average of  
    (individual 1)‡ 1,2,3)   1,2,3)   individuals 1,2,3)  
Th1 >Th2 GM-CSF 4.1 3.6 6.73 0.80 8.86 0.83 4.4 **
  TNF-α 12.3 6.2 2.91 0.28 4.40 0.92 2.8 **
  RANTES 3.1 4.0 1.23 0.72 3.88 0.71 6.3 **
  CCR1 5.1 122.0 7.18 0.85 12.73 1.95 46.7 **
  CCR2 8.9 30.7 7.57 0.55 10.95 2.10 10.4 **
  CCR5 1.9 109.0 10.64 0.46 15.08 1.92 21.7 **
  IκB 1.7 1.4 1.76 0.65 2.92 0.55 2.2 **
  TIMP-1 6.6 1.2 3.39 0.48 3.48 0.80 1.1 n.s.
  Caspase-1 2.9 1.9 8.08 0.72 8.87 0.75 1.7 **
  Clusterin 2.4 1.4 3.50 0.60 4.02 0.12 1.4 **
Th2 >Th1 IL-4R 7.0 2.3 7.90 0.48 6.31 1.01 3.0 n.s.
  FGFR 3.8 1.9 10.10 0.53 9.35 0.18 1.7 **
  CCR4 3.1 3.2 7.37 0.47 5.23 0.18 4.3 **
  STAT4 1.8 1.7 7.07 0.43 6.06 0.55 2.0 **
  1. †For calculations, see Materials and methods. ‡Same sample measured with both methods. §Data from Th1/Th2 culture samples representing three individuals were used. According to analysis of variance: n.s., not significant (p > 0.05); *significant (0.01 <p < 0.05); **highly significant (p < 0.01).
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Genome Biology

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