Figure 2

Multiple-tissue northern (MTN) blot analysis of SLC4A9 expression. (a,b) Hybridization with the SLC4A9 345F/R fragment as a probe. (c,d) Hybridization with the control β-actin cDNA as a probe. (a,c) MTN1; h, heart; br, brain; pl, placenta; lu, lung; lv, liver; sm, skeletal muscle; kid, kidney; pa, pancreas. (b,d) MTN2; sp, spleen; th, thymus; pr, prostate; te, testis; ov, ovary; int, small intestine; col, colon without mucosa; PBL, peripheral blood leukocytes. Not shown are the experiments with MTN3 (stomach, thyroid, spinal cord, lymph nodes, trachea, adrenal glands and bone marrow). No SLC4A9 expression was detected and no control hybridization was conducted on MTN3 (data not shown). (b) The entire blot is shown (reduced scale). (a,c,d) Signal areas only are shown. All MTN blots were obtained from Clontech.