Table 1 Primers used for construction of the deletion vectors and for PCR analysis
Different deletion constructs | ||||||
|---|---|---|---|---|---|---|
Primer | Sequence | pCE12 | pCE18 | pCE19 | pCE20 | α1-GFPtel- |
P4 | 5'-GGATCCATCAGATAACTCGCAAC | 5' | ||||
P548 | 5'-CTGCAGGTCGACTCTAGAGCTC | |||||
P7 | 5'-CAGATACAACGTCCCTCAAC | |||||
P8 | 5'-GCGGGATCCATCTTCATTTAAACTAGATG | 3' | 3' | 3' | ||
P9 | 5'-GGCTCGAGTTGCTACTCTCAGATATTC | |||||
P15 | 5'-CGGAGGTACCCCGATATTTAAAATCATTAATC | 3' | ||||
P20 | 5'-CCGCAGGATCCTTGAGAGTCTGCCATTTAAC | 5' | ||||
P23 | 5'-GTTAAATGGCAGACTCTCAAGAAGAAATGC | |||||
P25 | 5'-CCGGATCCAGACTTTCAGAGCGATATCTAG | 5' | ||||
P26 | 5'-GCGGATCCCTCTCAAAGTGTGACATATTCC | 5' | ||||
P27 | 5'-CGCAGGATCCAGAACAGTGGATTCGGAGGGAAATC | 5' | ||||
P28 | 5'-CCGAGGTACCGAGATAGAATCGATTAAATAATGGG | 3' | ||||
pGFP2 | 5'-CGCGAATTCTCACTTGTACAGCTCCTCCATG | |||||
pUCP1 | 5'-GTCGACCTGCAGGCATGCAAGCTT | |||||
pUCP2 | 5'-CGGGTACCGAGCTCGAATTC | |||||
pGEMP1 | 5'-CGCATGCTCCTCTAGACTCGAGGAATTCGG | |||||
pGEMP2 | 5'-GCTATGCATCCAACGCGTTGGGAGCTCTCC | |||||
PMUT2 | 5'-GGTCAGAAATACTAGTTGATTCAGATAAGAAAG | |||||