Figure 6From: Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutionsThe effect of protein concentration on background and detection limits. Six antigen mixes were added to fetal calf serum (FCS) solutions to produce a 10-fold and 100-fold increase in total protein concentration and a corresponding decrease in partial concentration of the antigens. After labeling, the mixes were analyzed with antibody microarrays. The images at the top of the figure present a detail from one of the arrays in each set. The median log10 of the Cy5/Cy3 fluorescence ratio is plotted as a function of the concentration ratio of the cognate antigen of the three indicated antibodies. The dashed line represents the log10 of the true ratio of antigen concentrations in the Cy5-labeled and Cy3-labeled solutions.Back to article page