Figure 2

Fluorescence dilution experiment at a lower concentration range. The second dilution experiment on glass, with much lower concentrations of dye used. (a) Cy3; (b) Cy5. The array design is a 2 x 5 grid of 8 x 8 patches. Each row in a patch is a serial dilution; thus the serial dilution has been repeated 80 times on this glass slide. The spots are about 400 μm apart and about 200 μm in diameter. The Cy3 image was scanned at a gain of 45 and the Cy5 image at a gain of 50, as commonly done for the Cy3 and Cy5 images in our laboratory.