Figure 6

Measurement of the configuration of tagged loci in homozygous plants from line EL702C. (a) A stacked image from a guard cell nucleus that contains three GFP spots was created. Scale bar = 5 μm. (b) Outline of nucleus (green wire-frame), position of the nucleolus (blue solid) and GFP fluorescence spots (red solids) were extracted from the stacked images, and a three-dimensional model was created. (c) The three-dimensional model was used to rotate the image by 90° in the y-axis, and the distance between the three GFP spots (a, b and c) was measured. Distance d_ab = 0.88 μm, d_bc= 1.13 μm, d_ac = 1.58 μm.