Figure 5

IPTG inhibition of mGFP5-LacI/NLS binding in nucleus. Homozygous plants of line EL702C were tested. Seedlings germinated on 0.5× MS agar plates for 10 days were transplanted onto 0.5× MS agar plates with and without 0.3 μM Dex. (a) Typical three-dimensional fluorescence images of nuclei from each treatment. (b) Graph of the observed number of spots and their percentages among total observed nuclei. (c) Levels of protein accumulation measured by western blot analysis using anti-GFP antibodies. M, protein size markers. Scale bar = 5 μm.