Figure 1

Identification of the Zap1p regulon. Zap1p target genes were identified using the predictions that they would: be expressed at higher levels in zinc-limited wild-type cells than in zinc-replete cells; expressed at higher levels in zinc-limited wild-type cells than in zinc-limited zap1 mutants; and contain one or more potential Zap1p-binding sites (zinc-responsive elements, ZREs) in their promoters. This gives 46 candidate Zap1p target genes; a further 65 may be indirect targets or lack the consensus ZRE. Adapted from [1].