Table 2 Primers used in the splicing analysis of USP25 and USP28
From: Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25
Primer | Sequence 5'-3' | Position in |
|---|---|---|
name | cDNA | |
(nucleotides) | ||
261F | GCCATGACCGTGGAGCAGAACG | 367-388 |
261R | CAGCACTAAACCAACAAGTATTGCCA | 891-916 |
1.2F | GAAGCCAGCATAGCAGAGAATAAAGC | 769-794 |
1.2R | CACAGGTGGTAATTCAGTAAACCAATG | 1,450-1,476 |
321F | GGGATGCACAACTTGCCCAG | 2,478-2,496 |
321R | CCCTGCTTCAGGGCCACACCTG | 2,772-2,793 |
N1 | AGGAGACCCAGAATAT | 2,574-2,589 |
121R | CAACCTTGCATATTCCAACT | 2,810-2,829 |
*5A11.1 | GCTGCTTTGGCATTCAGC | 1,947-1,968 |
*5A11.2 | TGACAAACTACCCTACTTCAATG | 2,804-2,882 |