Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans

Table 4 Sensitivity of RNAi by feeding versus injection

Gene(s)	Feeding phenotype	Injection phenotype	Gene information
K04G2.8a	Unc, Bmd, Lvl	Emb	apr-1 (APC-related)
F18C12.2a	Emb, Unc, Mlt, Bmd	Emb, Unc, Mlt, Lvl	DNA-J domain
ZK265.5,6^*	Gro	Gro	G-protein-coupled receptor/unknown function
T01G9.4	Emb, Gro, Clr	Emb, Gro	kup-2 (unknown function)
T01G9.5	Emb	Emb	mei-1 (meiotic spindle formation)
T01G9.6a	Emb, Gro, Pvl	Emb	kin-10 (CKII-beta subunit)
F52B5.6	Emb, Ste	Emb, Ste	Ribosomal protein L25
T19A6.2a	Gro	Gro	Ynr053p-like protein
D1081.2	Unc, Prz	Unc	MADS domain
D1081.8	Emb	Emb	Myb-like DNA-binding domain
K02B12.1	Unc, Mlt	Gro, Mlt, Lvl	ceh-6 (POU homeodomain protein)
K02B12.3	Ste, Gro	Ste	WD domains
K02B12.8	Him	None	Unknown function

From chromosome 1, 86 random genes from consecutive cosmids were selected to test the sensitivity of feeding versus injection for detecting RNAi phenotypes. Of these, 13 gave a phenotype by either method, 13 by feeding and 12 by injection; 11 of these genes have no previously described mutant or RNAi phenotype. Genes were determined to have a given phenotype if at least 10% of progeny had that phenotype, except for sterility, which required an average brood size of less than ten, and the Him phenotype, which required at least two out of three fed worms to have >5% male progeny. Bmd, body morphology defect; Clr, clear; Emb, embryonic lethal; Gro, slow growth; Him, high incidence of males; Lvl, larval lethal (death at any larval stage); Mlt, molting defect (old cuticle remains attached); Prz, paralyzed; Pvl, protruding vulva; Ste, sterile; Unc, uncoordinated. ^*The fragment used overlaps two predicted genes: ZK265.5 and ZK265.6.

ISSN: 1474-760X