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Table 4 Sensitivity of RNAi by feeding versus injection

From: Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans

Gene(s) Feeding phenotype Injection phenotype Gene information
K04G2.8a Unc, Bmd, Lvl Emb apr-1 (APC-related)
F18C12.2a Emb, Unc, Mlt, Bmd Emb, Unc, Mlt, Lvl DNA-J domain
ZK265.5,6* Gro Gro G-protein-coupled receptor/unknown function
T01G9.4 Emb, Gro, Clr Emb, Gro kup-2 (unknown function)
T01G9.5 Emb Emb mei-1 (meiotic spindle formation)
T01G9.6a Emb, Gro, Pvl Emb kin-10 (CKII-beta subunit)
F52B5.6 Emb, Ste Emb, Ste Ribosomal protein L25
T19A6.2a Gro Gro Ynr053p-like protein
D1081.2 Unc, Prz Unc MADS domain
D1081.8 Emb Emb Myb-like DNA-binding domain
K02B12.1 Unc, Mlt Gro, Mlt, Lvl ceh-6 (POU homeodomain protein)
K02B12.3 Ste, Gro Ste WD domains
K02B12.8 Him None Unknown function
  1. From chromosome 1, 86 random genes from consecutive cosmids were selected to test the sensitivity of feeding versus injection for detecting RNAi phenotypes. Of these, 13 gave a phenotype by either method, 13 by feeding and 12 by injection; 11 of these genes have no previously described mutant or RNAi phenotype. Genes were determined to have a given phenotype if at least 10% of progeny had that phenotype, except for sterility, which required an average brood size of less than ten, and the Him phenotype, which required at least two out of three fed worms to have >5% male progeny. Bmd, body morphology defect; Clr, clear; Emb, embryonic lethal; Gro, slow growth; Him, high incidence of males; Lvl, larval lethal (death at any larval stage); Mlt, molting defect (old cuticle remains attached); Prz, paralyzed; Pvl, protruding vulva; Ste, sterile; Unc, uncoordinated. *The fragment used overlaps two predicted genes: ZK265.5 and ZK265.6.