Alignments of complete amino acid sequences of Juan ORF1 and ORF2. (a) ORF1; (b) ORF2. Identical residues are in highlighted in black, conserved residues are shaded in dark gray, and similar residues are shaded in light gray. Alignments and shadings were performed with VectorNTI software. The sequence of Juan-A in Aedes aegypti is from accession number M95171, the sequence of Juan-C in Culex pipiens is from M91082, the sequence of JuanDm in D. melanogaster is from AC005452 (coordinates 3425-7660).