Figure 3

Structure of JuanDm elements in strain y; cn bw sp. (a) The full-size JuanDm element is represented as a white box, with two overlapping ORFs indicated as arrows. Positions of SpeI and ClaI restriction sites are shown and the black box above the element indicates the PCR fragment used as a probe in Figure 5a. Thick lines below represent the seven completely sequenced JuanDm elements found in Release 1. The internal deletion in element 5 is indicated as a gray region. (b) Sequences of integration sites of JuanDm elements shown in (a). Target site duplications are shown in bold and underlined. The 5'-most and 3'-most nucleotides of each element are shown in italics and separated by dots. For full-length elements, alignments in 5' region have been made to show variation in the 5' junction. The sequences of elements correspond to the following coordinates in release 1: 1, 13235891-13240123 in AE002787; 2, reverse complement of 7828981-7833212 in AE002566; 3, 4591855 4596087 in AE002593; 4, 268307-272538 in AE002629; 5, reverse complement of 7363833-7366823 in AE002708; 6, 874492-877244 in AE002638; 7, 2582603-2583169 in AE002602.