Table 2 Functional classification of cell-cycle-regulated transcripts found in Nb2 cells

Cell cycle: cyclins and cell-cycle regulators

   Transcripts that have a cell-cycle-modulated homolog in yeast

Cyclin E1 [78], peaks in G1

EGR-1, immediate-early gene (constitutive expression in Nb2 cells)

Cdc5-like protein [79], peaks in M

Cdk2, Cdk5 [78], peak in G1

   Cell-cycle-modulated transcripts with a yeast homolog (not modulated)

Cyclin B1, peaks in G2/M

   Cell-cycle-modulated transcripts with no yeast homolog

Cyclin B2, peaks in G2

Cyclin D2 [8], peaks in G1

Cyclin D3 [8], peaks in G1

Nucleotide metabolism, DNA replication and repair

Cdc21 homolog

Spermidine/spermine N-acetyl transferase (SSAT)

Ornithine decarboxylase (ODC) [80]

S-adenosylmethionine decarboxylase [80]

Prothymosin a

PARP-2 (Unknown 6-45)

Chromatin structure

Histones H2A, H2B, peak in S in mammals and in yeast

Cytoskeleton

Myosin heavy chain

Tubulin a, ß

ß-actin [6]

Clone 15 = rNUDC [81]

FAK p125

Cell-surface antigens, adhesion molecules and signaling molecules

(involved in apoptosis, survival and/or proliferation)

   Growth factor

FGF-2 [82]

   Surface molecules

Prolactin receptor Nb2 form [16]

T-cell receptor ? chain [17]

T-cell receptor a chain [17]

GnRH receptor [83]

Glucocorticoid receptor

Galectin-8

Leukocyte membrane glycoprotein, CD45

Vitamin D3 receptor

Thromboxane A2 receptor

ß₂-microglobulin

Cytoplasmic and/or nuclear signaling molecules

p38 Map kinase [21]

Pim-1 [9]

Gfi-1 [10]

Stathmin [84]

P13 kinase p110 a

Phospholipase C?1

14-3-3 ? and e

Bax

p53

RexB/NSP

Phosphatidylinositol transfer protein

a 4 phosphoprotein [79]

Transcription factors

IRF-1 [7]

c-Myc [18]

c-Fos (not in Nb2 cells [18])

MRG1-related protein

E2F-1 [78]

Zfx

Heat shock, stress response and chaperones

Cyclophilin (B)

TCP-1 e and ?

Hsp70 (not expressed in Nb2 cells)

Hsp70-like = Nb29 [41]

Hsp27

Hsp86

ß-actin

a₂-tubulin

Rdnuc (Golgi-associated protein) [11]

Myosin heavy chain

Focal adhesion kinase (FAK)

Metabolism (energy)

Phosphoglycerate kinase

Enolase a

Aldehyde dehydrogenase

ATP synthase ß subunit

Protein and RNA synthesis, modifications and degradation

   Ribosomal proteins

L3, L12, L13A, new ribosomal protein L15 type (unknown 4-27)

S8, S13

   Glycosylation factor

Itm1

   Elongation factor

EF-2 [77]

Inter-compartment transport and trafficking

CRM-1 = exportin 1

Sec-22

Glycine transporter

Unknown 4-58 (SH3, Rab GAP, TBC domain: putative nuclear pore protein)

Unknown function(s)

FGF-responsive Non/p54nrb

Unknowns T22, T34, 4-2, 4-4, 4-11, 4-15, 4-20, 4-49, 4-59, BO1

Unknowns 6-2, 6-3, 6-4, 6-9, 6-10, 6-12