Table 1 Differentially expressed transcripts found in Nb2 cells during cell-cycle progression using five different screening techniques

Differential display

   Unknown DD3

Three transcripts (2, 2.8, 3.7 kb)

expressed in G1 and G1/S,

(>4-fold induction)

Representational difference analysis: G1/S-GA

   ATP synthase ß subunit

Induced in G1/S, G2 (2-fold induction)

   Aldehyde dehydrogenase

Induced in late G1 (2-fold induction)

   Enolase a1

Induced in late G1 (2-fold induction)

   Dynein heavy chain

Induced in G1, G1/S (3-fold induction)

   TCP-1 e

Induced in G1/S, G2 (3-fold induction)

   aCOP (COPA)

Induced in late G1 (2-fold induction)

   Cdc21 homolog

Peaks in G1/S (>8-fold induction)

   Ribosomal protein L26

Induced in late G1 (2-fold induction)

   Itm1

Induced in G1/S (2-fold induction)

   Unknown T22

6 kb, 2-fold induction in G1/S

   Unknown T34

2 kb, 2-fold induction in G1/S

Subtractive suppressive hybridization: G1-GA

   Galectin-8

Induced in G1 (2-fold induction)

   Hsp86

Induced in late G1 (>3-fold induction)

   TCP-1 ?

Induced in late G1 (2-fold induction)

   Ribosomal protein L13A

Induced in late G1 (2-fold induction)

   Ribosomal protein L12

Induced in late G1 (2-fold induction)

   Ribosomal protein L3

Induced in G1 (2-fold induction)

Induced in G1 (2-fold induction)

   ß₂-microglobulin

Induced from late G1 to G2 (>5 fold induction)

   Adenine nucleotide translocator ANT-2

Induced in G1 (2-fold induction)

   Sec-22

Induced in G1 (2-fold induction)

   L-fucosidase

Induced in G1/S (>3-fold induction)

   CRM-1 homolog/exportin 1

Induced in G1/S (>3-fold induction)

   Ubiquitin/ribosomal protein S27a

Induced in G1/S (>3-fold induction)

   Ubiquitin/ribosomal protein S30 (FAU)

3-fold induction in G1 (1.5 kb)

   Unknown 4-2 (mouse selenoprotein R mRNA)

2-fold induction in G1/S (2 and 4 kb)

   Unknown 4-4 (human KIAA0081)

2-fold induction in G1 (1 kb)

   Unknown 4-11

4-fold induction in G1 (1 and 1.5 kb)

   Unknown 4-15

4-fold induction in G1 (2.5 kb)

   Unknown 4-16 (human ZNF207 or mouse Zep)

3-fold induction in G1 (1.5 kb)

   Unknown 4-20

2-fold induction in G1

   Unknown 4-27 (new ribosomal protein L15 type)

2-fold induction in G1

   Unknown 4-49

2-fold induction in G1

   Unknown 4-58 (SH3, Rab GAP, TBC domain)

2-fold induction in G1

   Unknown 4-59

Subtractive suppressive hybridization: GA-G1

   Spermidine/spermine N-acetyl transferase (SSAT)

Repressed transiently in G1

   Leukocyte common antigen (alternative splicing) CD45

Switch between two transcripts (one

repressed, the other induced in G1)

   ZFX

Repressed transiently in G1

   Ribosomal protein S8

Repressed transiently in G1

   Ribosomal protein S13

Repressed transiently in G1

   Unknown 6-2

Repressed transiently in G1

   Unknown 6-3 (hypothetical protein expressed in thymocytes)

Repressed transiently in G1

   Unknown 6-4

Repressed in G1

   Unknown 6-9

Repressed transiently in G1

   Unknown 6-10

Repressed transiently in G1

   Unknown 6-12 (human protein KIAA0710)

Repressed transiently in G1

   Unknown 6-45 (homolog to mouse PARP-2)

Repressed transiently in G1

Differential screening of a rat organized library: G2-GA

   Prothymosin a

Induced in G2

   Cyclophilin

Induced in G2

   ATP synthase ß subunit

Induced in G1/S and G2

   Tubulin a2

Induced in G1/S and G2

   GaPDH

Induced in G2

   Phosphoglycerate kinase

Induced in G1/S and G2

   MRG1 related protein

Induced in G1/S and G2

   Unknown BO1

Induced in G2

Analysis of weakly expressed candidate genes: UN, A, GI, G1/S, G2

Name

Kinetics

Ganglioside synthase (GD3)

P13 kinase

Phospholipase C?1

Bax

P53

FAKp125

14-3-3 e

14-3-3 ?

Vitamin D3 receptor

Glycine transporter

Thromboxane A2 receptor

Phosphatidylinositol transfer protein

RexB/NSP

Glucocorticoid receptor

Ga3PDH

Zif268 = EGR1