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Table 1 Differentially expressed transcripts found in Nb2 cells during cell-cycle progression using five different screening techniques

From: Analysis of prolactin-modulated gene expression profiles during the Nb2 cell cycle using differential screening techniques

Identity   Accession number Expression variations
Differential display    
   Unknown DD3   No EST Three transcripts (2, 2.8, 3.7 kb)
    expressed in G1 and G1/S,
    (>4-fold induction)
Representational difference analysis: G1/S-GA    
   ATP synthase ß subunit   M19044 Induced in G1/S, G2 (2-fold induction)
   Aldehyde dehydrogenase   U79118 Induced in late G1 (2-fold induction)
   Enolase a1   NM012554 Induced in late G1 (2-fold induction)
   Dynein heavy chain   D13896 Induced in G1, G1/S (3-fold induction)
   TCP-1 e   D43950(h) Induced in G1/S, G2 (3-fold induction)
   aCOP (COPA)   U24105 Induced in late G1 (2-fold induction)
   Cdc21 homolog   D26089 (m) Peaks in G1/S (>8-fold induction)
   Ribosomal protein L26   A1716351 Induced in late G1 (2-fold induction)
   Itm1   A1956728 (h) Induced in G1/S (2-fold induction)
   Unknown T22   A1053031 (h) 6 kb, 2-fold induction in G1/S
   Unknown T34   A1235326 2 kb, 2-fold induction in G1/S
Subtractive suppressive hybridization: G1-GA    
   Galectin-8   U09824 Induced in G1 (2-fold induction)
   Hsp86   X16857 Induced in late G1 (>3-fold induction)
   TCP-1 ?   AA900460 Induced in late G1 (2-fold induction)
   Ribosomal protein L13A   X68282 Induced in late G1 (2-fold induction)
   Ribosomal protein L12   AA900142 Induced in late G1 (2-fold induction)
   Ribosomal protein L3   A1687295 Induced in G1 (2-fold induction)
   Y00441 Induced in G1 (2-fold induction)
   ß2-microglobulin   D12771 Induced from late G1 to G2 (>5 fold induction)
   Adenine nucleotide translocator ANT-2   NM011342 (m) Induced in G1 (2-fold induction)
   Sec-22   NM012562 Induced in G1 (2-fold induction)
   L-fucosidase   U91538 Induced in G1/S (>3-fold induction)
   CRM-1 homolog/exportin 1   X81839 Induced in G1/S (>3-fold induction)
   Ubiquitin/ribosomal protein S27a   A47416 Induced in G1/S (>3-fold induction)
   Ubiquitin/ribosomal protein S30 (FAU)   AF195142 (m) 3-fold induction in G1 (1.5 kb)
   Unknown 4-2 (mouse selenoprotein R mRNA)   H35219 2-fold induction in G1/S (2 and 4 kb)
   Unknown 4-4 (human KIAA0081)   No EST 2-fold induction in G1 (1 kb)
   Unknown 4-11   AU035826 4-fold induction in G1 (1 and 1.5 kb)
   Unknown 4-15   AF046001 4-fold induction in G1 (2.5 kb)
   Unknown 4-16 (human ZNF207 or mouse Zep)   No EST 3-fold induction in G1 (1.5 kb)
   Unknown 4-20   AW435432 2-fold induction in G1
   Unknown 4-27 (new ribosomal protein L15 type)   A1121996 (m) 2-fold induction in G1
   Unknown 4-49   AW246248 (h) 2-fold induction in G1
   Unknown 4-58 (SH3, Rab GAP, TBC domain)   No EST 2-fold induction in G1
   Unknown 4-59    
Subtractive suppressive hybridization: GA-G1    
   Spermidine/spermine N-acetyl transferase (SSAT)   AA955996 Repressed transiently in G1
   Leukocyte common antigen (alternative splicing) CD45   Y00065 Switch between two transcripts (one
    repressed, the other induced in G1)
   ZFX   X75171 Repressed transiently in G1
   Ribosomal protein S8   AA874997 Repressed transiently in G1
   Ribosomal protein S13   L01123 Repressed transiently in G1
   Unknown 6-2   No EST Repressed transiently in G1
   Unknown 6-3 (hypothetical protein expressed in thymocytes)   AJ237585 (m) Repressed transiently in G1
   Unknown 6-4   No EST Repressed in G1
   Unknown 6-9   No EST Repressed transiently in G1
   Unknown 6-10   No EST Repressed transiently in G1
   Unknown 6-12 (human protein KIAA0710)   AB014610 (h) Repressed transiently in G1
   Unknown 6-45 (homolog to mouse PARP-2)   NM009632 (m) Repressed transiently in G1
Differential screening of a rat organized library: G2-GA    
   Prothymosin a   M86564 Induced in G2
   Cyclophilin   M19533 Induced in G2
   ATP synthase ß subunit   M19044 Induced in G1/S and G2
   Tubulin a2   AA686718 Induced in G1/S and G2
   GaPDH   X02231 Induced in G2
   Phosphoglycerate kinase   M31788 Induced in G1/S and G2
   MRG1 related protein   U65093 Induced in G1/S and G2
   Unknown BO1   No EST Induced in G2
Analysis of weakly expressed candidate genes: UN, A, GI, G1/S, G2
Name Accession number Fold induction Kinetics
Ganglioside synthase (GD3) D84068 > 4
P13 kinase D64045 > 3  
Phospholipase C?1 M34667 > 3  
Bax S76511 > 2
P53 X13058 > 2  
FAKp125 AF020777 > 3  
14-3-3 e M84416 > 2  
14-3-3 ? D17445 > 2
Vitamin D3 receptor J09838 3  
Glycine transporter M88595 3
Thromboxane A2 receptor D32080 2
Phosphatidylinositol transfer protein D17445 2
RexB/NSP U17604 > 2
Glucocorticoid receptor M14053 2
Ga3PDH J04147 2
Zif268 = EGR1 U75398 2
  1. Nb2 cells: UN, unsynchronized; GA, growth arrested; G1, G1 phase; G1/S, G1/S transition; G2, G2 phase. PC12, rat pheochromocytoma PC12 cells. 18S and 28 S, ribosomal RNA. Whenever possible, rat accession numbers (GenBank) are written in the second column. When this sequence is not in known for the rat, (h) or (m) indicates that the accession number corresponds, respectively, to a human and a mouse cDNA homologous to those of the rat. `No EST' means that our rat sequence does not correspond to any previously described EST in mammals. For some of the unknown cDNAs, an estimation of the size of the corresponding transcript(s) as well as the fold induction is given in the right column `Expression variations', and for weakly expressed candidate genes, the expression kinetics are shown.