Table 1 Oligonucleotide sequences of primers used

Anchored primer

   dT₁₂AA 5'-d(TTTTTTTTTTTTAA)-3'

   dT₁₂GA 5'-d(TTTTTTTTTTTTGA)-3'

   dT₁₂CA 5'-d(TTTTTTTTTTTTCA)-3'

   dT₁₂AG 5'-d(TTTTTTTTTTTTAG)-3'

   dT₁₂GG 5'-d(TTTTTTTTTTTTGG)-3'

   dT₁₂CG 5'-d(TTTTTTTTTTTTCG)-3'

   dT₁₂AC 5'-d(TTTTTTTTTTTTAC)-3'

   dT₁₂GC 5'-d(TTTTTTTTTTTTGC)-3'

   dT₁₂CC 5'-d(TTTTTTTTTTTTCC)-3'

   dT₁₂AT 5'-d(TTTTTTTTTTTTAT)-3'

   dT₁₂GT 5'-d(TTTTTTTTTTTTGT)-3'

   dT₁₂CT 5'-d(TTTTTTTTTTTTCT)-3'

Random primer

(arbitrary but defined sequence)

   P3 5'-d(GCCGTTCCAT)-3'

   R1 5'-d(GGAACTCCGT)-3'

   R2 5'-d(GGCAAGTCAC)-3'

   R4 5'-d(AGGACCGCTA)-3'

   R5 5'-d(CGGACCCCGG)-3'

   R7 5'-d(TACAACGAGG)-3'

   R8 5'-d(TGGATTGGTC)-3'

   R9 5'-d(TGGTAAAGGG)-3'

   R10 5'-d(TCGGTCATAG)-3'

   R11 5'-d(TACCTAAGCG)-3'

   R12 5'-d(CTGCTTGATG)-3'

   R14 5'-d(GATCGCATTG)-3'

   R18 5'-d(GGAACCAATC)-3'

   M2 5'-d(CACAGTTTGC)-3'

   M3 5'-d(CCACAGAGTA)-3'

   S 5'-d(GCGACCCATG)-3'

   A1 5'-d(ACAGAGCACA)-3'

   A2 5'-d(ACGTATCCAG)-3'

Oligo(dT) primer mix

(poly(A) anchored)

   MA Equal mix of dT₁₂AA, dT₁₂GA and dT₁₂CA

   MG Equal mix of dT₁₂AG, dT₁₂GG and dT₁₂CG

   MC Equal mix of dT₁₂AC, dT₁₂GC and dT₁₂CC

   MT Equal mix of dT₁₂AT, dT₁₂GT and dT₁₂CT

Extended random primer

   5'-d(GTCAGAATTC-random primer)-3'

Extended oligo(dT) primer

   5'-d(GTCAGAATTCT12)-3'