Fig. 3

In silico Pool Doublet Annotation and Method Performance. a The percent of singlets and doublets in the in -silico pools - separated by the number of multiplexed individuals per pool. b The percentage and number of doublets that are heterogenic (detectable by demultiplexing methods), heterotypic (detectable by doublet detecting methods), both (detectable by either method category) and neither (not detectable with current methods) for each multiplexed pool size. c Percent of droplets that each of the demultiplexing and doublet detecting methods classified correctly for singlets and doublet subtypes for different multiplexed pool sizes. d Matthew’s Correlation Coefficient (MCC) for each of the methods for each of the multiplexed pool sizes. e Balanced accuracy for each of the methods for each of the multiplexed pool sizes