Fig. 3
From: Enhancing prime editor flexibility with coiled-coil heterodimers
CC-PE performs prime editing in multiple mammalian cell lines. a Prime editing with sPE-P3P4, sPE-N5N6, sPE-ctrl, and unsplit PE in A549 cells. Nick gRNA was included in each PE system at DMD (+ 1 AC insertion). b Prime editing with sPE-P3P4, sPE-N5N6, sPE-ctrl, and unsplit PE in HCT116 cells. Nick gRNA was included in each PE system at DNMT1 (+ 1 ~ 15 ACAGGGGTGACGGGA deletion), EMX1 (+ 1 ~ 15 AGAAGGGCTCCCATC deletion), and DMD (+ 1 AC insertion). c Prime editing with sPE-P3P4, sPE-N5N6, sPE-ctrl, and unsplit PE in U2OS cells. Nick gRNA was included in each PE system at DNMT1 (+ 1 ~ 15 ACAGGGGTGACGGGA deletion), EMX1 (+ 1 ~ 15 AGAAGGGCTCCCATC deletion), and DMD (+ 1 AC insertion). Statistical significance in a-c was determined via unpaired t-tests (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns indicating not significant). Error bars indicated the mean ± standard deviation of at least three independent biological replicates