Fig. 2
From: Enhancing prime editor flexibility with coiled-coil heterodimers
CC-PE combining with the PE3 strategy enables precise genome editing. a Precise editing and undesired indel efficiencies of PE3, sPE-P3P4, sPE-N5N6, and sPE-ctrl at DMD (+ 1 AC insertion), FBN1 (+ 8 ~ 10 TCC deletion), DNMT1 (+ 1 ~ 15 ACAGGGGTGACGGGA deletion), RNF2 (+ 1 ~ 15 CTGAGGTGTTCGTTG deletion), EMX1 (+ 1 ~ 15 AGAAGGGCTCCCATC deletion), FBN1 (+ 2 C to A), IL2RB (+ 1 T to A, + 5 G to C), RUNX1 (+ 5 G to T), ALDOB (+ 5 G to A), FAM171B (+ 1 A to C), RIT1 (+ 4 G to A), EMX1 (+ 5 G to T), FANCF (+ 5 G to T), RNF2 (+ 5 G to T), RNF2 (+ 6 G to A), VEGFA (+ 5 G to T), HEXA (+ 5 G to C), and PRNP (+ 6 G to T) loci. b The heatmap generated by high-throughput sequencing shows average precise editing efficiencies of PE3, sPE-P3P4, sPE-N5N6, and sPE-ctrl among the 22 tested loci. Statistical significance in a was determined via unpaired t-tests (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns indicating not significant). Error bars indicated the mean ± standard deviation of at least three independent biological replicates