Fig. 5

UpSet plots summarizing the overlap in predictions between tools for the viral (A) and microbial (B) contigs from the seawater samples. The total number of identified viral contigs per tool is shown in the stacked bar plots on the left. Stacked bars above the upset plots visualize the number of viral contigs that were exclusively identified by each tool or tool combination. The left-most stacked bars show the number of contigs that were not identified as viruses by any of the tools. The CAT classification of the contigs is indicated as colors in the bar plots: blue represents contigs classified as viruses, orange represents contigs classified as “Bacteria,” “Archaea,” or “Eukaryota,” and gray represents “no support” or “nan” classifications. Heatmaps below and right of the upset plots visualize the frequency of viral (blue) or microbial (orange) hallmark genes (logarithmic arbitrary units, see the “Methods” section). The intensity of hallmark gene HMM profiles was determined by dividing the length sum of all the HMM hits by the contig length. Color of the tool names as in Fig. 1. For similar plots based on the soil and gut datasets, see Additional file 1: Figs. S5 and S6, respectively