Fig. 7
The function of CAMTA2 in transgenic pear callus and transgenic tomato plants. a Relative expression of the CAMTA2 gene in control and 5′-azacytidine (5′-Aza)-treated pear callus. b The CAMTA2-GFP fusion protein is localized to the nucleus of agroinfiltrated Nicotiana benthamiana leaf cells. c Growth of WT and CAMTA2-overexpressing (OE) pear callus. P1 = immediately after subculture, P2 = 14 days after subculture, and P3 = 24 days after subculture. d, e Cross-sections of pear callus stained with Toluidine Blue. The images show the number of cells in the same visual field of cross-sections of WT (d) and transgenic pear callus overexpressing CAMTA2 (e). Scale bars = 100 μm. f Growth status of T1-generation transgenic seedlings before transplanting. Scale bar = 1 cm. g Statistical analysis of root lengths of WT and T1-generation CAMTA2-OE seedlings. h Phenotypes of WT and CAMTA2-OE transgenic tomato plants. Scale bars = 1 cm. i Statistical analysis of plant height in the WT and transgenic tomato plants. j Representative phenotypes of WT CAMTA2-OE transgenic tomato fruits at 43, 46, 49, 52, 55, and 57 days after full bloom (DAFB). k Statistical analysis of fruit firmness in the WT and CAMTA2-OE transgenic tomato fruits harvested at the red stage (*P < 0.05; **P < 0.01; ***P < 0.001, two-tailed paired Student’s t-test)