Fig. 6

A hypermethylated DMR in the pear CAMTA2 gene. a GO enrichment analysis (top 15 significant terms) of hyper-CG-DMR-associated genes during pear domestication. b GO enrichment analysis (top 15 significant terms) of hyper-CG-DMR-associated genes during pear improvement. The blue stars represent the GO terms associated with senescence. c The CAMTA2 gene structure is shown at the top of the figure. Exons are represented by yellow-shaded boxes, introns are represented by black lines, and blue-shaded boxes represent the 5′ and 3′ UTRs. The bottom figure shows the CG methylation level of a DMR (Chr13:26,073,542–26,073,668) located in the CAMTA2 gene in the wild, landrace, and improved pear populations. The entire gene (2-kb upstream, gene body, and 2-kb downstream regions) is shown in the upper panel, and the DMR in exon 11 is shown enlarged below. d Comparison of the expression levels (FPKM) of CAMTA2 in the wild (yellow box), landrace (green box), and improved (blue box) pear populations (*P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant; two-tailed paired Student’s t-test)