Fig. 1
From: Divergent composition and transposon-silencing activity of small RNAs in mammalian oocytes
Small RNA composition and length distribution in oocytes from 12 representative vertebrate species. A Phylogenetic tree showing relationships between the 12 vertebrate species used in this study. The numbers on the tree indicate estimated divergence time in millions of years ago. B Small RNA composition and length distribution in oocytes of the 12 representative vertebrate species. C Sashimi plot of splice junctions in Dicer expressed in oocytes of mouse, rat, and golden hamster. The red asterisk indicates the RNA-seq signal of the MT-C element. IGV was used to compute the junction track from alignment data [38]. Junctions from the + strand are colored red and extend above the center line. Junctions from the − strand are blue and extend below the center line. Arc height and thickness are proportional to the depth of read coverage. D Detection of endogenous Dicer1 proteins in mouse, rat, and golden hamster MII oocytes via western blot. The black arrow indicates DicerS and the red arrowhead indicates DicerO. Dicer was detected using 100 mouse, 75 rat, and 44 golden hamster oocytes. Approximately 1 μg of total protein was used for NIH3T3 Dicer detection. DicerS, somatic Dicer1; DicerO, oocyte Dicer1; M, marker